The microsomal glucose-6-phosphatase enzyme of pancreatic islets
نویسندگان
چکیده
منابع مشابه
The microsomal glucose-6-phosphatase enzyme of pancreatic islets.
Microsomal fractions isolated from pancreatic islet cells were shown to contain high specific glucose-6-phosphatase activity. The islet-cell glucose-6-phosphatase enzyme has the same Mr (36,500), similar immunological properties and kinetic characteristics to the hepatic microsomal glucose-6-phosphatase enzyme.
متن کاملThermal stability of microsomal glucose-6-phosphatase.
The thermal stability of glucose-6-phosphatase in rat liver microsomes was examined in untreated and cholate-treated microsomes. Activity of the enzyme was measured with both glucose-6-P and mannose-6-P as substrates. Heat treatment did not cause glucose-6-phosphatase activity to decline to zero with a single rate constant in untreated microsomes. Instead, heat treatment produced an enzyme with...
متن کاملDetermination of liver microsomal glucose-6-phosphatase.
A procedure for the determination of liver microsomal glucose-6-phosphatase is described. Homogenization and ultracentrifrigation were used to prepare a precipitate whose character was defined by monitoring the desire enzyme activity which serves as a marker. Activity of the enzyme was determined by means of a sensitive colorimetric reaction for the product, inorganic phosphate. Non-enzymatic h...
متن کاملLiver Microsomal Glucose 6 - Phosphatase , Inorganic Pyrophosphatase , and Pyrophosphate - Glucose
Recently, we (1) described experiments which strongly supported the common identity of glucose 6-phosphatase,’ inorganic pyrophosphatase, pyrophosphate-glucose phosphotransferase, and mannose B-phosphate-glucose phosphotransferase activities present in a preparation obtained by ammonium sulfate fractionation of deoxycholate-dispersed rat liver microsomes. Previously, Segal, Washko, and Lee (2, ...
متن کاملThe ontogeny of human hepatic microsomal glucose-6-phosphatase proteins.
We have studied 250 human liver biopsy samples to determine the ontogeny of the microsomal glucose-6-phosphatase (EC 3.1.3.9) system. Human hepatic glucose-6-phosphatase enzyme activity develops at 11 weeks' gestation and slowly increases to approximately 10% of adult activity at term. In the first week after birth, activity rises to adult values. Increases in enzyme activity coincide with incr...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1988
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2550471